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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
23/10/2009 |
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Data da última atualização: |
01/03/2010 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
ANDREOTTI, R.; MATOS, M. de F. C.; GONÇALVES, K. N.; OSHIRO, L. M.; LIMA-JUNIOR, M. S. da C.; PAIVA, F.; LEITE, F. L. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; MARIA DE FÁTIMA CEPA MATOS, UFMS; KELLY NODA GONÇALVES, UFMS; LEANDRA MARLA OSHIRO, UFMS; MANOEL SEBASTIÃO DA COSTA LIMA-JUNIOR, UFMS; FERNANDO PAIVA, UFMS; FÁBIO LEIVAS LEITE, UFPel. |
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Título: |
Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Revista Brasileira de Parasitologia Veterinária, São Paulo, v.18, n.2, p.20-24, abr-jun. 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG
tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep. |
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Palavras-Chave: |
NcSRS2; Proteína recombinante; Teste. |
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Thesagro: |
Diagnostico; Elisa; Neospora Caninum; Neosporose; Ovino; Sanidade Animal. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02075naa a2200301 a 4500
001 1573041
005 2010-03-01
008 2009 bl --- 0-- u #d
100 1 $aANDREOTTI, R.
245 $aComparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep.
260 $c2009
520 $aNeospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep.
650 $aDiagnostico
650 $aElisa
650 $aNeospora Caninum
650 $aNeosporose
650 $aOvino
650 $aSanidade Animal
653 $aNcSRS2
653 $aProteína recombinante
653 $aTeste
700 1 $aMATOS, M. de F. C.
700 1 $aGONÇALVES, K. N.
700 1 $aOSHIRO, L. M.
700 1 $aLIMA-JUNIOR, M. S. da C.
700 1 $aPAIVA, F.
700 1 $aLEITE, F. L.
773 $tRevista Brasileira de Parasitologia Veterinária, São Paulo$gv.18, n.2, p.20-24, abr-jun. 2009.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro completo
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Biblioteca(s): |
Catálogo Coletivo de Periódicos Embrapa; Embrapa Agroindústria de Alimentos; Embrapa Amapá; Embrapa Amazônia Oriental. |
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Identificador: |
191 |
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Data corrente: |
09/05/2002 |
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Data da última atualização: |
05/05/2015 |
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Código do título: |
1850001 |
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ISSN: |
0001-9704 |
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Código CCN: |
001824-4 |
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Título e Subtítulo: |
AFINIDAD |
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Entidade: |
Instituto Quimico de Sarria |
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Local de publicação: |
Barcelona, Espanha, ES |
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Periodicidade: |
Bimestral |
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Inicio de publicação: |
1924 |
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Coleções da unidade: |
Embrapa Agroindústria de Alimentos
1953 (30); 1954 31(133,135-144); 1955 32(145-156); 1956 33(157-158); 1966 23(241-246); 1967 24(147-252); 1968 25(253-262); 1969 26(263-272); 1970 27(273-282); 1971 28(283-292); 1972 29(293-302); 1973 30(303-312); 1974 31(313-313-314,316-322); 1975 32(323-332); 1976 33(333-342); 1977 34(343-352); 1978 35(353-358); 1979 36(359-364); 1980 37(365-370); 1981 38(371-376); 1982 39(377-382); 1983 40(383-388); 1984 41(389-394); 1985 42(395)
Classificação: 540.5
Embrapa Amapá
1979 36(360-361,363-364)
Embrapa Amazônia Oriental
1950 (95-96); 1951 (97-108); 1952 (109-120); 1953 (121-132); 1954 (133-144); 1955 (145-156); 1956 (157-168); 1957 (169-180); 1958 (181-182); 1959 (193-204); 1960 17(205-210); 1961 18(211-216); 1962 19(217-222); 1963 20(223-228); 1964 21(229-232,234); 1965 22(235-240); 1966 23(241-246) nesp; 1967 24(247-252); 1968 25(253-262); 1969 26(263-272); 1970 27(273-275,277-282); 1971 28(283-292); 1972 29(293-302); 1973 30(303-312); 1974 31(313-322); 1975 32(323-332); 1976 33(333-342); 1977 34(343-352); 1978 35(353-354); 1979 35(355-358), 36(359-364); 1980 37(365-370); 1981 38(371-376), 39(376); 1982 39(377-382); 1983 40(383-387); 1984 41(389-394); 1985 42(395-400); 1986 43(401-406); 1987 44(407-412); 1988 45(413-418); 1989 46(419-424); 1990 47(425-427,430); 1991 48(431-436); 1992 49(437-442); 1993 50(443,445,447-448); 1994 51(449-454); 1995 52(455-460); 1996 53(461-466) nesp; 1997 54(467-469); 1999 56(480-484); 2000 57(485-490); 2001 58(491-496); 2002 59(497-502); 2003 60(503-508); 2004 61(509,511-514); 2005 62(516-517,519-520); 2006 63(521,523-526); 2007 64(527-529,531-532); 2008 65(533-536)
Classificação: 540.5A257 |
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